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rat monoclonal anti ifn gamma  (R&D Systems)


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    R&D Systems rat monoclonal anti ifn gamma
    Rat Monoclonal Anti Ifn Gamma, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+mouse+ifn+%CE%B3/Mouse+IFN-gamma+Antibody/pmc12583703__41467_2025_64730_MOESM2_ESM-235-0-5
    Average 94 stars, based on 26 article reviews
    rat monoclonal anti ifn gamma - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: EZH2 elicits CD8 + T-cell desert in esophageal squamous cell carcinoma via suppressing CXCL9 and dendritic cells.
    Article Snippet: To measure mouse mature DCs, the antibody combination of rabbit anti-CXCL9 (1:100, bs2551R, Bioss, Beijing, China) or rabbit anti-CXCR7 (1:100, bs-1305R, Bioss, Beijing, China) with Armenian hamster anti-mouse CD11c (1:100, 14- 0114-82, Thermo Fisher Scientific, CA, USA) was used to incubate with the sections overnight at 4°C and CoraLite594-conjugated goat anti-rabbit IgG (H+ L) (1:200, SA00013-4, Proteintech, Wuhan, China) and goat antiArmenian hamster IgG HL(Alexa Fluor488) (1:200, ab173003, Abcam, Cambridge, MA, USA) were used as secondary antibodies. .. To determine mouse cytotoxic CD8+ T cells, rat anti-mouse IFN-γ (1:100, MAB485-SP, R&D systems, MN, USA) combined with rabbit anti-CD8A antibody (1:100, NBP2-29475, Novus biologicals, Colorado, USA) was used as the primary antibodies and incubated with the sections overnight at 4°C. .. After three washes with PBS, Rhodamine (TRITC)-goat anti-rat IgG (H+ L) (1:100, SA00007-11, Proteintech, Wuhan, China) and Coralite488conjugated goat anti-rabbit IgG(H+ L) (1:500, SA00013-1, Proteintech, Wuhan,China)were incubatedwith the sections at 37°C for 30min.

    Article Title: EZH2 elicits CD8 + T-cell desert in esophageal squamous cell carcinoma via suppressing CXCL9 and dendritic cells
    Article Snippet: To measure mouse mature DCs, the antibody combination of rabbit anti-CXCL9 (1:100, bs-2551R, Bioss, Beijing, China) or rabbit anti-CXCR7 (1:100, bs-1305R, Bioss, Beijing, China) with Armenian hamster anti-mouse CD11c (1:100, 14-0114-82, Thermo Fisher Scientific, CA, USA) was used to incubate with the sections overnight at 4°C and CoraLite594-conjugated goat anti-rabbit IgG (H + L) (1:200, SA00013-4, Proteintech, Wuhan, China) and goat anti-Armenian hamster IgG HL(Alexa Fluor488) (1:200, ab173003, Abcam, Cambridge, MA, USA) were used as secondary antibodies. .. To determine mouse cytotoxic CD8 + T cells, rat anti-mouse IFN-γ (1:100, MAB485-SP, R&D systems, MN, USA) combined with rabbit anti-CD8A antibody (1:100, NBP2-29475, Novus biologicals, Colorado, USA) was used as the primary antibodies and incubated with the sections overnight at 4°C. .. After three washes with PBS, Rhodamine (TRITC)-goat anti-rat IgG (H + L) (1:100, SA00007-11, Proteintech, Wuhan, China) and Coralite488-conjugated goat anti-rabbit IgG(H + L) (1:500, SA00013-1, Proteintech, Wuhan, China) were incubated with the sections at 37°C for 30 min.



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    Immunization of the ICD dying tumor cells activates Th1 cells in tumor microenvironment. A . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). B . IFNγ and IL-4 in in the axillary LNs (L) of different groups ( n = 5) by ELISA. C . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). D . <t>IFN-γ</t> and IL-4 in tumor tissues (M) of different groups by ELISA. E-H . Flow cytometry analysis for the content and distribution of immune cells in tumor tissues as indicated. ** p < 0.01, *** p < 0.001, **** p < 0.0001
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    Immunization of the ICD dying tumor cells activates Th1 cells in tumor microenvironment. A . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). B . IFNγ and IL-4 in in the axillary LNs (L) of different groups ( n = 5) by ELISA. C . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). D . IFN-γ and IL-4 in tumor tissues (M) of different groups by ELISA. E-H . Flow cytometry analysis for the content and distribution of immune cells in tumor tissues as indicated. ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Journal: Journal of Cancer Research and Clinical Oncology

    Article Title: Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis

    doi: 10.1007/s00432-025-06087-z

    Figure Lengend Snippet: Immunization of the ICD dying tumor cells activates Th1 cells in tumor microenvironment. A . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). B . IFNγ and IL-4 in in the axillary LNs (L) of different groups ( n = 5) by ELISA. C . Schema of the immunization experiment to isolate lymphocytes from tumor tissue for analysis ( n = 5). D . IFN-γ and IL-4 in tumor tissues (M) of different groups by ELISA. E-H . Flow cytometry analysis for the content and distribution of immune cells in tumor tissues as indicated. ** p < 0.01, *** p < 0.001, **** p < 0.0001

    Article Snippet: All antibody information is shown following: PE Rat Anti-CD11b (553311, BD), Alexa Fluor ® 647 Rat Anti-Mouse F4/80 (565854, BD) BD HorizonTM PE-CF594 Rat Anti-Mouse IFN-γ antibody (5562333 BD).

    Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry